Journal: Communications Biology

Article Title: The ROS–FOXO pathway mediates broad-spectrum detoxification of acaricides in Tetranychus cinnabarinus

doi: 10.1038/s42003-025-08726-0

Figure Lengend Snippet: a The O 2 • − content after fenpropathrin, cyflumetofen and chlorpyrifos treatment in T. cinnabarinus . Acetone was used for the control treatment, and the data was normalized accordingly. Data was expressed as the mean, n = 3. b DHE fluorescence signal intensity in T. cinnabarinus after DHE staining. Acetone was used for the control treatment. Data was expressed as the mean, n = 17–34. In a , b lowercase letters indicate statistically significant differences at different time points following treatment with the same acaricide. In contrast, uppercase letters represent significant differences between different acaricides, as analyzed by two-way ANOVA (Tukey’s HSD), p < 0.05. The F value represents the result of the Fisher test. c T. cinnabarinus were sectioned after DHE staining, and images were obtained using a laser confocal microscope. The scale bar represents 50 μm. The O 2 • − -specific fluorescent probe is shown in red. In the figure, the deep red signal indicates a higher ROS level.

Article Snippet: Two hundred 3–5-day-old adult female mites were homogenized in precooled PBS, followed by the addition of O 2 • − probe dihydroethidium (DHE) (Yeasen Biotechnology Co., Ltd., Shanghai, China) to achieve a final concentration of 30 μM .

Techniques: Control, Fluorescence, Staining, Microscopy

Journal: Nature Communications

Article Title: Stress-trained microalgae robots with probiotics backpack and intestinal brake for inflammatory bowel disease management

doi: 10.1038/s41467-025-66692-x

Figure Lengend Snippet: A Schematic illustration on the treatment protocol of DSS-induced acute colitis mice. B Curve chart variations of murine body weight of the six groups, normalized to the percentage of day 0 body weight ( n = 5 mice). C Curve chart analysis on the DAI scores of treated mice ( n = 5 mice). D Photographs of murine colons after oral treatment with different formulations. E Histogram analysis on the colon length of treated mice ( n = 5 mice). F Histological images of H&E-stained sections (scale bar, 200 μm) (black arrows: intact crypt structure, yellow arrows: mucosal epithelium cell shedding, blue arrows: inflammatory cell infiltration, and red arrows: loss of crypt and goblet cells). G CD86 staining (M1-type macrophages) and CD206 staining (M2-type macrophages) on the colons of treated mice. The scale bar, 200 μm. H , I The level of MDA and the activity of SOD in the collected serum of treated mice ( n = 5 mice). J DHE staining on the colons of treated mice. The scale bar, 200 μm. K–M Levels of TNF-α, IL-6, and IL-1β in colonic tissues isolated from different groups ( n = 5 mice). N Immunofluorescence images of Occludin and ZO-1-stained colon tissues. The scale bar, 200 μm. All values were presented as means ± SD. All statistical analyses were conducted by using one-way ANOVA.

Article Snippet: Dihydroethidium (DHE) probe was provided by US EVERBRIGHT Biotechnology Co., Ltd (Suzhou, China).

Techniques: Staining, Activity Assay, Isolation, Immunofluorescence